Protocols

  • Xenopus 基因组数据参考网站

    Major web resource sites for Xenopus genomic data:Institute Tool URLBroad Institute Integrative Genomics Viewer http://www.broadinstitute.org/software/igv/ Crick Institute UCSC browser http://gen

    2018/08/02

  • labs using Xenopus as a model system

    Hollis Cline Lab Neural circuit development and plasticityKurt Haas Lab Neural circuit developmentDominique Alfandari ADAM metalloprotease function during embryonic Development. UM

    2018/07/22

  • Usefull links for Xenopus resources

    Xenbase: http://www.xenbase.org/entry/ The Transcriptome of Xenopus Tropicalis:   http://jason.chuang.ca/research/xenopus/refseq.html Frog Development: https://embryology.med.unsw.edu.au/embryology/index.php/Frog_Development

    2018/07/22

  • 4%多聚甲醛的配制方法

    4%多聚甲醛的配制 1000 ml 4%多聚甲醛1)称取40g多聚甲醛;2)加入温热(60~70℃)的蒸馏水约450ml,加入1N NaOH溶液适量,搅拌至透明;3)加入0.2M的PBS 500 ml,调节pH值至7.2-7.4,PBS的终浓度为0.1 M;4)蒸馏水定容至1000 ml。滤纸过虑,所得到的4%多聚甲醛可以保存在室温或4度。 注意要点:1.加NaOH助溶时宜少量

    2018/07/08

  • in situ hybridization

    in situ hybridization - Harland , R.M. 1991. Meth. Cell Biol. 36:685. The first time you use a particular probe it is worthwhile to preabsorb it. This takes about 24 h and dramatically

    2018/07/08

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